General description Third Edition of this laboratory reference covers the most advanced instrumentation and techniques in the field and features over 200 step-by-step protocols. It includes an expanded FISH chapter; more information and protocols for breakage studies; up-to-the-minute coverage of computer imaging, regulation, the molecular aspects of leukemias, and chromosome spreading; and listings of Internet resources as well as the latest on cell culture principles; peripheral blood cytogenetic methods; prenatal chromosomal diagnosis; the preparation of chromosome stains; the analysis of cytogenetic abnormalities; the cytogenetic analysis of hematologic malignant diseases, and much more! Table of Contents 1. The Cell and Cell Division 2. Cytogenetics: An Overview 3.

1. Osu Cytogenetics Lab

Peripheral Blood Cytogenetic Methods 4. General Cell Culture Principles and Fibroblast Culture 5. Prenatal Chromosome Diagnosis 6. Chromosome Stains 7. Cytogenetic Analysis of Hematologic Malignant Diseases 8. Cytogenetic Methods and Findings in Human Solid Tumors 9. Microscopy and Imaging 10.

Computer Image Processing 11. Chromosome Analysis 12. Fragile X and Other Heritable Fragile Sites on Human Chromosomes 13. Molecular Cytogenetics: Definitions, Clinical Aspects, and Protocols 14. Spectral Karyotyping 15.

Laboratory Safety, Quality Control, and Regulations.

Cytogenetics is the study of chromosome morphology, structure, pathology, function, and behavior. The field has evolved to embrace molecular cytogenetic changes, now termed cytogenomics. Cytogeneticists utilize an assortment of procedures to investigate the full complement of chromosomes and/or a targeted region within a specific chromosome in metaphase or interphase. Tools include routine analysis of G-banded chromosomes, specialized stains that address specific chromosomal structures, and molecular probes, such as fluorescence in situ hybridization (FISH) and chromosome microarray analysis, which employ a variety of methods to highlight a region as small as a single, specific genetic sequence under investigation. The AGT Cytogenetics Laboratory Manual, Fourth Edition offers a comprehensive description of the diagnostic tests offered by the clinical laboratory and explains the science behind them. One of the most valuable assets is its rich compilation of laboratory-tested protocols currently being used in leading laboratories, along with practical advice for nearly every area of interest to cytogeneticists. In addition to covering essential topics that have been the backbone of cytogenetics for over 60 years, such as the basic components of a cell, use of a microscope, human tissue processing for cytogenetic analysis (prenatal, constitutional, and neoplastic), laboratory safety, and the mechanisms behind chromosome rearrangement and aneuploidy, this edition introduces new and expanded chapters by experts in the field.

Osu Cytogenetics Lab

Some of these new topics include a unique collection of chromosome heteromorphisms; clinical examples of genomic imprinting; an example-driven overview of chromosomal microarray; mathematics specifically geared for the cytogeneticist; usage of ISCN’s cytogenetic language to describe chromosome changes; tips for laboratory management; examples of laboratory information systems; a collection of internet and library resources; and a special chapter on animal chromosomes for the research and zoo cytogeneticist. The range of topics is thus broad yet comprehensive, offering the student a resource that teaches the procedures performed in the cytogenetics laboratory environment, and the laboratory professional with a peer-reviewed reference that explores the basis of each of these procedures. This makes it a useful resource for researchers, clinicians, and lab professionals, as well as students in a university or medical school setting.